Pectin methyl esterase from Aspergillus aculeatus: expression cloning in yeast and characterization of the recombinant enzyme.

Seventeen full-length cDNAs encoding pectin methyl esterase I (PME I) have been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. Yeast colonies expressing functional PME I were identified on agar plates containing highly esterified pectin, and a cDNA encodin...

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Main Authors: Christgau, S, Kofod, L V, Halkier, T, Andersen, L N, Hockauf, M, Dörreich, K, Dalbøge, H, Kauppinen, S
格式: Artigo
語言:Inglês
出版: 1996
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1217846/
https://ncbi.nlm.nih.gov/pubmed/8920970
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