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Pectin methyl esterase from Aspergillus aculeatus: expression cloning in yeast and characterization of the recombinant enzyme.
Seventeen full-length cDNAs encoding pectin methyl esterase I (PME I) have been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. Yeast colonies expressing functional PME I were identified on agar plates containing highly esterified pectin, and a cDNA encodin...
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Auteurs principaux: | , , , , , , , |
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Format: | Artigo |
Langue: | Inglês |
Publié: |
1996
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Sujets: | |
Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1217846/ https://ncbi.nlm.nih.gov/pubmed/8920970 |
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