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Structural environment of an essential cysteine residue of xylanase from Chainia sp. (NCL 82.5.1).

N-(2,4-Dinitroanilino)maleimide (DAM) reacts covalently with the thiol group of the xylanase from Chainia leading to complete inactivation in a manner similar to N-ethylmaleimide, but provides a reporter group at the active site of the enzyme. Increasing amounts of xylan offered enhanced protection...

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Bibliografiske detaljer
Main Authors: Rao, M, Khadilkar, S, Bandivadekar, K R, Deshpande, V
Format: Artigo
Sprog:Inglês
Udgivet: 1996
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1217417/
https://ncbi.nlm.nih.gov/pubmed/8670151
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