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Reversibility of biotin-binding by selective modification of tyrosine in avidin.

The tight interaction between the vitamin biotin and the protein avidin is so strong (Ka approximately 10(15) M-1) that conditions which are usually sufficient for protein denaturation fail to dissociate the avidin-biotin complex. In order to form a reversible interaction between the two biomolecule...

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Dettagli Bibliografici
Autori principali: Morag, E, Bayer, E A, Wilchek, M
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1996
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1217322/
https://ncbi.nlm.nih.gov/pubmed/8645205
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