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Ability of methotrexate to inhibit translocation to the cytosol of dihydrofolate reductase fused to diphtheria toxin.

A fusion protein consisting of dihydrofolate reductase and diphtheria toxin A-fragment was made by genetically linking cDNA for the two proteins followed by in vitro transcription and translation in a rabbit reticulocyte lysate system. The dihydrofolate reductase in the fusion protein exhibited enzy...

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Detalles Bibliográficos
Autores principales: Klingenberg, O, Olsnes, S
Formato: Artigo
Lenguaje:Inglês
Publicado: 1996
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1216956/
https://ncbi.nlm.nih.gov/pubmed/8573105
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