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Temperature-Sensitive Mutants for the Replication of Plasmids in ESCHERICHIA COLI I. Isolation and Specificity of Host and Plasmid Mutations

Temperature-sensitive mutants of Escherichia coli defective in the replication of the plasmid colicinogenic factor E1 (ColE(1)) were isolated following mutagenesis of E. coli K12 strain carrying the ColE(1) factor. Following the mutagenic treatment an enrichment procedure utilizing the replacement o...

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Main Authors: Kingsbury, David T., Helinski, Donald R.
格式: Artigo
語言:Inglês
出版: 1973
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1212933/
https://ncbi.nlm.nih.gov/pubmed/4576229
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