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A comparison of methods for the isolation and fractionation of reticulocyte ribosomes

1. Polysomes, ribosomes and pH5 enzymes were isolated from rabbit reticulocytes by acidifying the post-mitochondrial supernatant to pH6·0 to precipitate all ribonucleoprotein particles and about half the pH5 enzymes; the precipitate was redissolved in buffer, pH7·6, and fractionated by zone centrifu...

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Detalhes bibliográficos
Main Authors: Arnstein, H. R. V., Cox, R. A., Gould, H., Potter, H.
Formato: Artigo
Idioma:Inglês
Publicado em: 1965
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1207066/
https://ncbi.nlm.nih.gov/pubmed/4953780
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