Detection of Isoniazid-Resistant Mycobacterium tuberculosis Strains by a Multiplex Allele-Specific PCR Assay Targeting katG Codon 315 Variation

We describe a simple multiplex allele-specific (MAS)-PCR assay to detect mutations in the second base of the katG gene codon 315, including AGC→ACC and ACA (Ser→Thr) substitutions that confer resistance to isoniazid (INH) in Mycobacterium tuberculosis clinical isolates. The 315 ACC allele is found i...

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Veröffentlicht in:	J Clin Microbiol
Hauptverfasser:	Mokrousov, Igor, Otten, Tatiana, Filipenko, Maxim, Vyazovaya, Anna, Chrapov, Eugeny, Limeschenko, Elena, Steklova, Lidia, Vyshnevskiy, Boris, Narvskaya, Olga
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2002
Schlagworte:	Mycobacteriology and Aerobic Actinomycetes
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120554/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12089271/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.7.2509-2512.2002
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