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Detection of Methicillin-Resistant Staphylococcus aureus and Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real-Time PCR

A molecular assay for the simultaneous detection of a Staphylococcus aureus-specific gene and the mecA gene, responsible for the resistance to methicillin in staphylococci, was evaluated. The assay included an automated DNA extraction protocol conducted with a MagNA Pure instrument and real-time PCR...

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Библиографические подробности
Главные авторы: Grisold, Andrea J., Leitner, Eva, Mühlbauer, Gerhard, Marth, Egon, Kessler, Harald H.
Формат: Artigo
Язык:Inglês
Опубликовано: American Society for Microbiology 2002
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC120553/
https://ncbi.nlm.nih.gov/pubmed/12089253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.40.7.2392-2397.2002
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