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Direct Cloning of Yeast Genes from an Ordered Set of Lambda Clones in Saccharomyces Cerevisiae by Recombination in Vivo

We describe a technique that facilitates the isolation of yeast genes that are difficult to clone. This technique utilizes a plasmid vector that rescues lambda clones as yeast centromere plasmids. The source of these lambda clones is a set of clones whose location in the yeast genome has been determ...

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Bibliografiska uppgifter
Huvudupphovsmän: Erickson, J. R., Johnston, M.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1993
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1205418/
https://ncbi.nlm.nih.gov/pubmed/8514124
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