Promoters of the Broad Host Range Plasmid Rk2: Analysis of Transcription (Initiation) in Five Species of Gram-Negative Bacteria

A broad host range cloning vector was constructed, suitable for monitoring promoter activity in diverse Gram-negative bacteria. This vector, derived from plasmid RSF1010, utilized the firefly luciferase gene as the reporter, since the assay for its bioluminescent product is sensitive, and measuremen...

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Bibliographic Details
Main Authors: Greener, A., Lehman, S. M., Helinski, D. R.
Format: Artigo
Language:Inglês
Published: 1992
Subjects:
Investigations
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1204802/
https://ncbi.nlm.nih.gov/pubmed/1732166
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