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Use of Pulsed Field Gel Electrophoresis and Transposon Mutagenesis to Estimate the Minimal Number of Genes Required for Motility in Caulobacter Crescentus

To facilitate the mapping of transposon insertion mutations in Caulobacter crescentus, we have used pulsed field gel electrophoresis to construct a detailed physical and genetic map of the C. crescentus genome. Restriction fragments were generated by DraI, AseI, or SpeI which cleave the C. crescentu...

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Detalhes bibliográficos
Main Authors: Ely, B., Ely, T. W.
Formato: Artigo
Idioma:Inglês
Publicado em: 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1203877/
https://ncbi.nlm.nih.gov/pubmed/2558955
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