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Transposon Tn5 Target Specificity: Preference for Insertion at G/C Pairs

The procaryotic transposon Tn5 inserts into many different sites within a single gene, but some sites (hotspots) are targeted repeatedly. Hotspots are not closely related in sequence, but most have G/C pairs at the ends of the nine base pairs duplicated by Tn5 insertion. In pBR322, the major hotspot...

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Autores principales: Lodge, J. K., Weston-Hafer, K., Berg, D. E.
Formato: Artigo
Lenguaje:Inglês
Publicado: 1988
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1203542/
https://ncbi.nlm.nih.gov/pubmed/2852135
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