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Mutations That Improve the Binding of Yeast Flp Recombinase to Its Substrate

When yeast FLP recombinase is expressed from the phage λ P(R) promoter in a Salmonella host, it cannot efficiently repress an operon controlled by an operator/promoter region that includes a synthetic, target FLP site. On the basis of this phenotype, we have identified four mutant FLP proteins that...

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Autores principales: Lebreton, B., Prasad, P. V., Jayaram, M., Youderian, P.
Formato: Artigo
Lenguaje:Inglês
Publicado: 1988
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1203293/
https://ncbi.nlm.nih.gov/pubmed/3284782
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