Lysis with Saponin improves detection of the response through CD203c and CD63 in the basophil activation test after crosslinking of the high affinity IgE receptor FcεRI

BACKGROUND: The basophil activation test (BAT), in which translocation of markers to the surface of blood basophils is measured in response to allergen by flow cytometry, is a rapid assay that is gaining popularity. Two markers are currently being evaluated for the BAT; CD63 and the lineage-specific...

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Bibliografiska uppgifter
Huvudupphovsmän: Hoffmann, Hans Jürgen, Bøgebjerg, Mette, Nielsen, Lars Peter, Dahl, Ronald
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2005
Ämnen:
Research
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1201566/
https://ncbi.nlm.nih.gov/pubmed/15996266
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1476-7961-3-10
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