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Mutation of the Zinc-Binding Metalloprotease Motif Affects Bacteroides fragilis Toxin Activity but Does Not Affect Propeptide Processing

To evaluate the role of the zinc-binding metalloprotease in Bacteroides fragilis toxin (BFT) processing and activity, the zinc-binding consensus sequences (H348, E349, H352, G355, H358, and M366) were mutated by site-directed-mutagenesis. Our results indicated that single point mutations in the zinc...

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Autori principali: Franco, Augusto A., Buckwold, Simy L., Shin, Jai W., Ascon, Miguel, Sears, Cynthia L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2005
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1201259/
https://ncbi.nlm.nih.gov/pubmed/16041055
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.73.8.5273-5277.2005
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