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Evaluation of a Novel Heminested PCR Assay Based on the Phosphoglucosamine Mutase Gene for Detection of Helicobacter pylori in Saliva and Dental Plaque

A novel heminested PCR protocol was developed for the specific detection of Helicobacter pylori at low copy numbers. A set of primers specific for the phosphoglucosamine mutase gene (glmM) of H. pylori produced a 765-bp fragment that was used as template for the heminested primer pair delineating a...

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Publicat a:J Clin Microbiol
Autors principals: Goosen, C., Theron, J., Ntsala, M., Maree, F. F., Olckers, A., Botha, S. J., Lastovica, A. J., van der Merwe, S. W.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2002
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC120087/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11773117/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.40.1.205-209.2002
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