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Influence of Specimen Age and Use of Different Negative Controls in Determination of Intracytoplasmic Levels of Cytokines after Whole-Blood Culture Assay

Intracytoplasmic detection of cytokines by flow cytometry has become a powerful tool in the characterization of cytokine-producing cells. However, it is not known to what extent specimen age and the use of various negative controls may influence the amount of cytokine-positive cells. We therefore co...

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Détails bibliographiques
Auteurs principaux: Schultz, Christian, Rott, Christina, Temming, Petra, von Puttkammer, Julia, Bucsky, Peter
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2002
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC119920/
https://ncbi.nlm.nih.gov/pubmed/11874866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CDLI.9.2.295-298.2002
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