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Studies on alkaline phosphatase. Phosphorylation of calf intestinal alkaline phosphatase by (32)P-labelled pyrophosphate

1. A purified preparation of alkaline phosphatase from calf-intestinal mucosa was phosphorylated by (32)P-labelled PP(i) at a serine residue on the enzyme. Under the conditions employed, up to 0·15μm-labelled sites were obtained from 1μm-[(32)P]PP(i). 2. The phosphorylated enzyme was labile, the rat...

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Detalhes bibliográficos
Main Authors: Fernley, H. N., Bisaz, Sylvia
Formato: Artigo
Idioma:Inglês
Publicado em: 1968
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1198654/
https://ncbi.nlm.nih.gov/pubmed/4295986
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