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Characterization of the Type III restriction endonuclease PstII from Providencia stuartii

A new Type III restriction endonuclease designated PstII has been purified from Providencia stuartii. PstII recognizes the hexanucleotide sequence 5′-CTGATG(N)(25-26/27-28)-3′. Endonuclease activity requires a substrate with two copies of the recognition site in head-to-head repeat and is dependent...

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Main Authors: Sears, Alice, Peakman, Luke J., Wilson, Geoffrey G., Szczelkun, Mark D.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2005
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1192830/
https://ncbi.nlm.nih.gov/pubmed/16120967
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gki787
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