Purification and characterization of a protease from Actinobacillus pleuropneumoniae serotype 1, an antigen common to all the serotypes.

A high molecular-mass proteolytic enzyme of Actinobacillus pleuropneumoniae serotype 1, was purified from culture supernatants (CSN) by using DEAE-cellulose and sepharose-4B-gelatin chromatography. In 10% SDS-polyacrylamide gels copolymerized with porcine gelatin, the protease showed a single band o...

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Bibliografiske detaljer
Main Authors: Negrete-Abascal, E, Tenorio, V R, Guerrero, A L, García, R M, Reyes, M E, de la Garza, M
Format: Artigo
Sprog:Inglês
Udgivet: 1998
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1189474/
https://ncbi.nlm.nih.gov/pubmed/9684047
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