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Experimental conditions affecting ribonucleic acid polymerase in isolated rat liver nuclei. Effect of nucleoside triphosphate concentration, temperature, ammonium sulphate and heparin

1. The conditions affecting the activity of RNA polymerase in isolated rat liver nuclei were studied with Mg(2+) or Mn(2+) as activating ions. 2. The enzyme assayed with Mg(2+) and at low ionic strength is saturated by a lower concentration of nucleotide substrates than if assayed with Mn(2+) at low...

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Détails bibliographiques
Auteurs principaux: Novello, F., Stirpe, F.
Format: Artigo
Langue:Inglês
Publié: 1969
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1187776/
https://ncbi.nlm.nih.gov/pubmed/5821730
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