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PCR amplification introduces errors into mononucleotide and dinucleotide repeat sequences

The polymerase chain reaction (PCR) is used universally for accurate exponential amplification of DNA. We describe a high error rate at mononucleotide and dinucleotide repeat sequence motifs. Subcloning of PCR products allowed sequence analysis of individual DNA molecules from the product pool and r...

詳細記述

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書誌詳細
主要な著者: Clarke, L A, Rebelo, C S, Gonçalves, J, Boavida, M G, Jordan, P
フォーマット: Artigo
言語:Inglês
出版事項: British Medical Journal Publishing Group 2001
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1187094/
https://ncbi.nlm.nih.gov/pubmed/11577179
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