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Amplification of PCR products in excess of 600 base pairs using DNA extracted from decalcified, paraffin wax embedded bone marrow trephine biopsies

Aims—To establish a robust method of extracting DNA from paraffin wax embedded bone marrow trephine (PBMT) biopsies for the amplification of relatively long polymerase chain reaction (PCR) products. Method—Xylene and ethanol were used to remove paraffin wax from eight formalin fixed, EDTA decalcifie...

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書誌詳細
主要な著者: Wickham, C L, Boyce, M, Joyner, M V, Sarsfield, P, Wilkins, B S, Jones, D B, Ellard, S
フォーマット: Artigo
言語:Inglês
出版事項: British Medical Journal Publishing Group 2000
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オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1186897/
https://ncbi.nlm.nih.gov/pubmed/10884917
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