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Biosynthesis of thiamine. Incorporation experiments with (14)C-labelled substrates and with [(15)N]glycine in Saccharomyces cerevisiae
1. Yeast was grown in a minimal synthetic medium together with a range of (14)C-labelled substrates under standardized conditions. After isolation, the purified thiamine was cleaved by sulphite and the pyrimidine and thiazole moieties were purified and assayed for radioactivity. 2. In order of decre...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1968
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1186770/ https://ncbi.nlm.nih.gov/pubmed/5679359 |
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