A simple assay for adenylate cyclase in intact cells by affinity elution chromatography.

A method using the principle of affinity elution chromatography is described for the assay of adenylate cyclase in intact human platelets. By incubating platelet-rich plasma in the presence of radioactively labelled adenine, the ATP pool of the cells was prelabelled. Formation of labelled cyclic AMP...

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Bibliographic Details
Main Authors: Sinha, A K, Colman, R W
Format: Artigo
Language:Inglês
Published: 1978
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1185972/
https://ncbi.nlm.nih.gov/pubmed/215121
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