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Deoxyribonucleic acid–ribonucleic acid hybridization. Annealing and quantitative recovery of intact ribosomal ribonucleic acid molecules from hybrids

A simple and efficient method for hybridization and subsequent recovery of non-fragmented ribosomal RNA from the hybrid is described. The procedure involves annealing of immobilized denatured DNA bound on cellulose nitrate membrane filters to complementary RNA in 50% (v/v) formamide–0·33m-potassium...

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Detalhes bibliográficos
Main Authors: Fry, Michael, Artman, Michael
Formato: Artigo
Idioma:Inglês
Publicado em: 1969
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1185101/
https://ncbi.nlm.nih.gov/pubmed/4907881
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