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Kinetic studies with the use of proton-magnetic-resonance spectroscopy of the specific alpha-deuteration of amino acids by Escherichia coli aspartate aminotransferase.

Escherichia coli aspartate aminotransferase was exposed to aspartate or phenylalanine without oxo acid in buffered 2H2O. The alpha-hydrogen of the amino acids underwent first-order exchange with respect to both substrate and enzyme. P.m.r. spectroscopy gave consistent reaction-rate constants. The de...

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Detalhes bibliográficos
Main Authors: Gout, E, Chesne, S, Beguin, C G, Pelmont, J
Formato: Artigo
Idioma:Inglês
Publicado em: 1978
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1184019/
https://ncbi.nlm.nih.gov/pubmed/352342
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