Structure-Based in Vitro Engineering of the Anthranilate Synthase, a Metabolic Key Enzyme in the Plant Tryptophan Pathway

Rice (Oryza sativa) anthranilate synthase α-subunit, OASA2, was modified by in vitro mutagenesis based on structural information from bacterial homologs. Twenty-four amino acid residues, predicted as putative tryptophan binding sites or their proximal regions in the OASA2 sequence, were selected and...

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Bibliographische Detailangaben
Hauptverfasser: Kanno, Takuya, Komatsu, Akira, Kasai, Koji, Dubouzet, Joseph G., Sakurai, Minako, Ikejiri-Kanno, Yasuko, Wakasa, Kyo, Tozawa, Yuzuru
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society of Plant Biologists 2005
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1183412/
https://ncbi.nlm.nih.gov/pubmed/16040654
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.105.062885
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