Genetic Diversity of Viable, Injured, and Dead Fecal Bacteria Assessed by Fluorescence-Activated Cell Sorting and 16S rRNA Gene Analysis

A novel approach combining a flow cytometric in situ viability assay with 16S rRNA gene analysis was used to study the relationship between diversity and activity of the fecal microbiota. Simultaneous staining with propidium iodide (PI) and SYTO BC provided clear discrimination between intact cells...

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Detalhes bibliográficos
Main Authors: Ben-Amor, Kaouther, Heilig, Hans, Smidt, Hauke, Vaughan, Elaine E., Abee, Tjakko, de Vos, Willem M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Microbial Ecology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1183343/
https://ncbi.nlm.nih.gov/pubmed/16085863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.8.4679-4689.2005
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