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Development of PCR Assays Targeting Genes in O-Antigen Gene Clusters for Detection and Identification of Escherichia coli O45 and O55 Serogroups

The Escherichia coli O45 O-antigen gene cluster of strain O45:H2 96-3285 was sequenced, and conventional (singleplex), multiplex, and real-time PCR assays were designed to amplify regions in the wzx (O-antigen flippase) and wzy (O-antigen polymerase) genes. In addition, PCR assays targeting the E. c...

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Detalhes bibliográficos
Main Authors: DebRoy, Chitrita, Fratamico, Pina M., Roberts, Elisabeth, Davis, Michael A., Liu, Yanhong
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1183307/
https://ncbi.nlm.nih.gov/pubmed/16085897
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.8.4919-4924.2005
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