Minimal cross-recombination between wild-type and loxP511 sites in vivo facilitates truncating both ends of large DNA inserts in pBACe3.6 and related vectors

Contrary to several earlier reports, we find that cross-recombination between wild-type and the mutant loxP511 sites is <0.5% of that between two wild-type sites if Cre protein is expressed by phage P1 during an infection. The finding enabled us to develop a procedure to truncate DNA progressivel...

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Hlavní autoři: Shakes, Leighcraft A., Garland, Douglas M., Srivastava, Deepak K., Harewood, Ken R., Chatterjee, Pradeep K.
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2005
Témata:
Methods Online
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1182172/
https://ncbi.nlm.nih.gov/pubmed/16061933
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni119
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