Fluorescence Resonance Energy Transfer Detection of Synaptophysin I and Vesicle-associated Membrane Protein 2 Interactions during Exocytosis from Single Live Synapses

To investigate the molecular interactions of synaptophysin I and vesicle-associated membrane protein 2 (VAMP2)/synaptobrevin II during exocytosis, we have used time-lapse videomicroscopy to measure fluorescence resonance energy transfer in live neurons. For this purpose, fluorescent protein variants...

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Bibliographic Details
Main Authors: Pennuto, Maria, Dunlap, David, Contestabile, Andrea, Benfenati, Fabio, Valtorta, Flavia
Format: Artigo
Language:Inglês
Published: The American Society for Cell Biology 2002
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC117936/
https://ncbi.nlm.nih.gov/pubmed/12181340
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E02-01-0036
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