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Study of the thermal denaturation of ribonuclease A by differential thermal analysis and susceptibility to proteolysis

1. The thermally induced change in conformation of ribonuclease A in solution was investigated by differential thermal analysis and the susceptibility of the enzyme to proteolytic digestion by ficin. 2. A transition with a mid-point of 60.5°C at pH4.2 was observed directly by differential thermal an...

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Autori principali: Winchester, B. G., Mathias, A. P., Rabin, B. R.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1970
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1178862/
https://ncbi.nlm.nih.gov/pubmed/5463263
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