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The effects of glucagon and insulin on adenosine 3′:5′-cyclic monophosphate concentrations in an organ culture of mature rat liver

1. A modified radioimmunoassay for cyclic AMP was developed from the method of Steiner et al. (1969). Cyclic [(3)H]AMP was used as the radioactive tracer. Free and antibody-bound nucleotides were separated by adsorption of protein to Millipore filters. The assay was used to measure amounts of cyclic...

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Библиографические подробности
Главные авторы: Siddle, Kenneth, Kane-Maguire, Barbara, Campbell, Anthony K.
Формат: Artigo
Язык:Inglês
Опубликовано: 1973
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC1177651/
https://ncbi.nlm.nih.gov/pubmed/4352833
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