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Reactions of d-glyceraldehyde 3-phosphate dehydrogenase with chromophoric thiol reagents

The kinetics of the reaction of d-glyceraldehyde 3-phosphate dehydrogenase with 5,5′-dithiobis-(2-nitrobenzoic acid) show that NAD(+) dissociates from the enzyme before the reaction. In contrast 2-chloromercuri-4-nitrophenol reacts with the holoenzyme without prior dissociation of NAD(+). These stud...

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Detalhes bibliográficos
Main Authors: Harrigan, P. J., Trentham, D. R.
Formato: Artigo
Idioma:Inglês
Publicado em: 1971
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1177227/
https://ncbi.nlm.nih.gov/pubmed/4332544
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