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pH-dependence and structure–activity relationships in the papain-catalysed hydrolysis of anilides

The pH-dependence of the Michaelis–Menten parameters for the papain-catalysed hydrolysis of N-acetyl-l-phenylalanylglycine p-nitroanilide was determined. The equilibrium binding constant, K(s), is independent of pH between 3.7 and 9.3, whereas the acylation constant, k(+2), shows bell-shaped pH-depe...

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Detalhes bibliográficos
Main Authors: Lowe, G., Yuthavong, Y.
Formato: Artigo
Idioma:Inglês
Publicado em: 1971
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1177120/
https://ncbi.nlm.nih.gov/pubmed/5126467
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