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The purification and characterization of acetoacetyl-coenzyme A reductase from Azotobacter beijerinckii

A soluble acetoacetyl-CoA reductase (EC 1.1.1.36) was purified 54-fold from Azotobacter beijerinckii N.C.I.B. 9067 and the reaction product identified as d(−)-β-hydroxybutyryl-CoA. The Michaelis constants for acetoacetyl-CoA, NADPH and NADH were determined and the reaction rate was found to be some...

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Detalhes bibliográficos
Main Authors: Ritchie, G. A. F., Senior, P. J., Dawes, E. A.
Formato: Artigo
Idioma:Inglês
Publicado em: 1971
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1176571/
https://ncbi.nlm.nih.gov/pubmed/4398981
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