Cloning, expression, and characterization of a nitric oxide synthase protein from Deinococcus radiodurans

We cloned, expressed, and characterized a hemeprotein from Deinococcus radiodurans (D. radiodurans NO synthase, deiNOS) whose sequence is 34% identical to the oxygenase domain of mammalian NO synthases (NOSoxys). deiNOS was dimeric, bound substrate Arg and cofactor tetrahydrobiopterin, and had a nor...

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Main Authors: Adak, Subrata, Bilwes, Alexandrine M., Panda, Koustubh, Hosfield, David, Aulak, Kulwant S., McDonald, John F., Tainer, John A., Getzoff, Elizabeth D., Crane, Brian R., Stuehr, Dennis J.
格式: Artigo
語言:Inglês
出版: The National Academy of Sciences 2002
主題:
Biological Sciences
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC117522/
https://ncbi.nlm.nih.gov/pubmed/11756668
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.012470099
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