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Purification and properties of 2-furoyl-coenzyme A hydroxylase from Pseudomonas putida F2

1. 2-Furoyl-CoA hydroxylase of Pseudomonas putida F2 has been purified 60-fold by a combination of (NH(4))(2)SO(4) fractionation, DEAE-cellulose chromatography and agarose chromatography. 2. The purified enzyme catalyses the formation of 5-hydroxy-2-furoyl-CoA, which tautomerizes to form 5-oxo-Δ(2)-...

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Bibliografiska uppgifter
Huvudupphovsmän: Kitcher, J. P., Trudgill, P. W., Rees, J. S.
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1972
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Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1174307/
https://ncbi.nlm.nih.gov/pubmed/4655411
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