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Pseudouridylation of yeast U2 snRNA is catalyzed by either an RNA-guided or RNA-independent mechanism

Yeast U2 small nuclear RNA (snRNA) contains three pseudouridines (Ψ35, Ψ42, and Ψ44). Pus7p and Pus1p catalyze the formation of Ψ35 and Ψ44, respectively, but the mechanism of Ψ42 formation remains unclear. Using a U2 substrate containing a single (32)P radiolabel at position 42, we screened a GST-O...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Ma, Xiaoju, Yang, Chunxing, Alexandrov, Andrei, Grayhack, Elizabeth J, Behm-Ansmant, Isabelle, Yu, Yi-Tao
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2005
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1173158/
https://ncbi.nlm.nih.gov/pubmed/15962000
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.emboj.7600718
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