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An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants
BACKGROUND: Traditional gene replacement procedures are still time-consuming. They usually necessitate cloning of the gene to be mutated, insertional inactivation of the gene with an antibiotic resistance cassette and exchange of the plasmid-borne mutant allele with the bacterial chromosome. PCR and...
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| Main Authors: | , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
BioMed Central
2005
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1173109/ https://ncbi.nlm.nih.gov/pubmed/15907219 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-5-30 |
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