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The purification in high yield and characterization of rat hepatic glucokinase.

A new improved procedure for the purification of rat hepatic glucokinase (ATP-d-glucose 6-phosphotransferase, EC 2.7.1.2) is given. A key step is affinity chromatography on Sepharose-N-(6-aminohexanoyl)-2-amino-2-deoxy-d-glucopyranose. A homogeneous enzyme, specific activity 150 units/mg of protein,...

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Autors principals: Holroyde, M J, Allen, M B, Storer, A C, Warsy, A S, Chesher, J M, Trayer, I P, Cornish-Bowden, A, Walker, D G
Format: Artigo
Idioma:Inglês
Publicat: 1976
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1172582/
https://ncbi.nlm.nih.gov/pubmed/1275894
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