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Investigation of ribonuclease-catalysed kinetics by a micro-calorimetric method.

A rapid micro-calorimetric method for the simultaneous determination of the Michaelis-Menten parameters and the enthalpy of enzymic reactions is developed. The hydrolysis of 2': 3'-cyclic CMP by ribonuclease A is studied to test the proposed method; values obtained are in good agreement wi...

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Detalhes bibliográficos
Main Authors: Tribout, M, Paredes, S, Léonis, J
Formato: Artigo
Idioma:Inglês
Publicado em: 1976
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1172544/
https://ncbi.nlm.nih.gov/pubmed/1259718
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