A new way to rapidly create functional, fluorescent fusion proteins: random insertion of GFP with an in vitro transposition reaction

BACKGROUND: The jellyfish green fluorescent protein (GFP) can be inserted into the middle of another protein to produce a functional, fluorescent fusion protein. Finding permissive sites for insertion, however, can be difficult. Here we describe a transposon-based approach for rapidly creating libra...

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Pubblicato in:	BMC Neurosci
Autori principali:	Sheridan, Douglas L, Berlot, Catherine H, Robert, Antoine, Inglis, Fiona M, Jakobsdottir, Klara B, Howe, James R, Hughes, Thomas E
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	BMC 2002
Soggetti:	Methodology Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC117241/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12086589/ https://ncbi.nlm.nih.govhttps://doi.org/10.1186/1471-2202-3-7
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