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Mutants of Tn3 resolvase which do not require accessory binding sites for recombination activity.
Tn3 resolvase promotes site-specific recombination between two res sites, each of which has three resolvase dimer-binding sites. Catalysis of DNA-strand cleavage and rejoining occurs at binding site I, but binding sites II and III are required for recombination. We used an in vivo screen to detect r...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1999
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1171230/ https://ncbi.nlm.nih.gov/pubmed/10064606 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/18.5.1407 |
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