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Wild-type Flp recombinase cleaves DNA in trans.
Site-specific recombinases of the Integrase family utilize a common chemical mechanism to break DNA strands during recombination. A conserved Arg-His-Arg triad activates the scissile phosphodiester bond, and an active-site tyrosine provides the nucleophile to effect DNA cleavage. Is the tyrosine res...
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
1999
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1171171/ https://ncbi.nlm.nih.gov/pubmed/9927438 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/18.3.784 |
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