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Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins.
ER-to-Golgi transport in yeast may be reproduced in vitro with washed membranes, purified proteins (COPII, Uso1p and LMA1) and energy. COPII coated vesicles that have budded from the ER are freely diffusible but then dock to Golgi membranes upon the addition of Uso1p. LMA1 and Sec18p are required fo...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1998
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1170560/ https://ncbi.nlm.nih.gov/pubmed/9545229 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/17.8.2156 |
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