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Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing.
In order to assess the role of Prp22 in yeast pre-mRNA splicing, we have purified the 130 kDa Prp22 protein and developed an in vitro depletion/reconstitution assay. We show that Prp22 is required for the second step of actin pre-mRNA splicing. Prp22 can act on pre-assembled spliceosomes that are ar...
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| Main Authors: | , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1998
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1170553/ https://ncbi.nlm.nih.gov/pubmed/9524130 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/17.7.2086 |
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