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Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing.

In order to assess the role of Prp22 in yeast pre-mRNA splicing, we have purified the 130 kDa Prp22 protein and developed an in vitro depletion/reconstitution assay. We show that Prp22 is required for the second step of actin pre-mRNA splicing. Prp22 can act on pre-assembled spliceosomes that are ar...

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Bibliografiske detaljer
Main Authors: Schwer, B, Gross, C H
Format: Artigo
Sprog:Inglês
Udgivet: 1998
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1170553/
https://ncbi.nlm.nih.gov/pubmed/9524130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/17.7.2086
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