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Prp22, a DExH-box RNA helicase, plays two distinct roles in yeast pre-mRNA splicing.

In order to assess the role of Prp22 in yeast pre-mRNA splicing, we have purified the 130 kDa Prp22 protein and developed an in vitro depletion/reconstitution assay. We show that Prp22 is required for the second step of actin pre-mRNA splicing. Prp22 can act on pre-assembled spliceosomes that are ar...

詳細記述

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書誌詳細
主要な著者: Schwer, B, Gross, C H
フォーマット: Artigo
言語:Inglês
出版事項: 1998
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC1170553/
https://ncbi.nlm.nih.gov/pubmed/9524130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/17.7.2086
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