Improved and Versatile Transformation System Allowing Multiple Genetic Manipulations of the Hyperthermophilic Archaeon Thermococcus kodakaraensis

We have recently developed a gene disruption system for the hyperthermophilic archaeon Thermococcus kodakaraensis by utilizing a pyrF-deficient mutant, KU25, as a host strain and the pyrF gene as a selectable marker. To achieve multiple genetic manipulations for more advanced functional analyses of...

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Detalhes bibliográficos
Main Authors: Sato, Takaaki, Fukui, Toshiaki, Atomi, Haruyuki, Imanaka, Tadayuki
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1169065/
https://ncbi.nlm.nih.gov/pubmed/16000802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.7.3889-3899.2005
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